Can we develop new biomanufacturing technologies to reduce the dependence on the chemical industry?
The answer is yes!
Using the "Next Generation Industrial Biotechnology" system accumulated and successfully developed by Tsinghua University in the past 30 years, PhaBuilder’s technical team has carried out series transformations on the basis of the halophilic bacteria- originally found in Ayding Lake, Xinjiang/China, to make it more suitable for industrial mass production. Since then, the technical team of Tsinghua University and PhaBuilder have continued to focus on the development of bacterial strains. Since 2018, the bacterial strains have been iterated to 20 generations. Now PhaBuilder can use a new metabolic pathway to produce PHB, P34HB, P34HBHV, PHBHHx, PHB5HV, P3HP3HB and other products.
The R&D and mass production history of the R&D team that founded PhaBuilder——
2009-2012
Two halophilic bacteria, Halomonas TD01 and LS21, were successfully screened in Aiding Lake, Xinjiang
Developed a series of halophilic bacteria-based tools
2013-2014
The laboratory completed the open fermentation production of PHB and PHBV (TD01)
LS21 open continuous fermentation for 65 days
Further increase in PHA production
2015-2017
The production strain of P34HB was successfully developed and the small test was completed
Successfully realized the pilot test of P34HB product 5 tons fermenter
2017-2018
Successful development of a rapid gene editing tool for halophilic bacteria
High cell density PHB was successfully mass-produced in a 5-ton fermenter (cell dry weight above 100g/L, PHA content above 95%)
Propose the theory of "Next Generation Bioindustrial Technology (NGIB)" to reduce the cost of biomanufacturing
2019-Now
Low-salt strains were successfully developed and verified by small scale scale-up
P34HB mass production capacity reaches 1,000 tons
Core Technology
Excellent NGIB Platform with Halomonas
New factory of cell
Halomonas cell chassis, open incubation, no sterilization, significantly reduces energy cost
Synthetic Biology Technology Platform
The development of gene editing and metabolic network regulation tools significantly improve the efficiency of strain modification and optimization
Different PHA products
A wide variety of PHA materials can be provided
High value compound production platform
It can produce ectoine, phloroglucinol and other promising high-value compounds
Chassis Bacteria
Revolutionary discovery and modification of new Chassis Bacteria
PhaBuilder’s technical team screened the most adaptable industrial microbial
strain Halomonas, a brand-new extreme chassis halophilic bacteria, from Ayding Lake(Xinjiang/China), one of
the hottest and driest regions in the world with a salt concentration of 200g / L. After years of genetic
modification by the technical team, the production efficiency of synthetic biology related products have
been greatly improved. Performance advantages: save resources in production, improve production efficiency,
not compete with human food resources and the ability to produce a variety of synthetic biological products from
one bacteria.
Genetic Modification
Make chassis bacteria more powerful
The technical team of Tsinghua University and PhaBuilder have added and optimized many excellent
performances for the bacterial strains. Through continuous iteration, PhaBuilder has accumulated reusable
engineering transformation experience and has been successfully applied to mass production.
■ Reconstitute the substrate metabolic pathway of the Halomonas chassis cell ,
enabling the utilization of multiple substrates. ■ Promoting engineering and hemoglobin configuration can improve the efficiency of oxygen utilization hundreds of times. ■ Rearrange metabolic pathways of population effect. ■ Use morphological engineering to change the
growth mode of bacteriat. ■ Modify the surface charge of the halophilic bacteria to enable self-flocculation, which is conducive to continuous fermentation and bacterial liquid separation.. ■ Open fermentation reduces the complexity of
process flow and equipment.
Bisection becomes multiple fission
Biological Circuits: Designing and Building a Gene Expression Oscillator for Microbial
Growth
Self sedimentation
Intellectual Property
Covering the whole process of metabolic regulation, fermentation production, and efficient purification
PhaBuilder has always been committed to new technology innovation in synthetic biology and green manufacturing of biological products. Using the "Next Generation Industrial Biotechnology" platform accumulated and successfully developed by Tsinghua University in the past 30 years, PhaBuilder’s technical team has carried out series transformations on the basis of the halophilic bacteria- originally found in Ayding Lake, Xinjiang/China, to make it more suitable for industrial mass production. The team has completed bench-scale production of 5 and 15 tons in 2015-2021, and completed the production of multiple PHAs in 200 tons fermenters in 2021-2022. The technical team of Tsinghua University and PhaBuilder have continued to focus on the development of bacterial strains. Since 2018, the bacterial strains have been iterated to 20 generations. Now PhaBuilder can use a new metabolic pathway to produce PHB, P34HB, P34HBHV, PHBHHx, PHB5HV, P3HP3HB and other products. PhaBuilder has dozens of core invention patents in key areas. In addition to PHAs, it also covers products such as cadaverine, ectoine, inositol, amino acids, and 3-hydroxypropionate.
Two halophilic bacteria, Halomonas TD01 and LS21, were successfully screened in Aiding Lake, Xinjiang
Developed a series of halophilic bacteria-based tools
